A Conductive Nanowire-Mesh Biosensor for Ultrasensitive Detection of Serum C-Reactive Protein in Melanoma

Zuan Tao Lin, Yaxi Li, Jianhua Gu, Huie Wang, Zhuan Zhu, Xia Hong, Zijing Zhang, Qinqin Lu, Jingyi Qiu, Xifan Wang, Jiming Bao, Tianfu Wu

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Detection of extracutaneous melanoma is still challenging and is of importance in improving survival rate. In this report, an ultrasensitive biosensor is constructed where a C-reactive protein (CRP) aptamers based molecular recognition core and a conductive polypyrrole (PPy) nanowire mesh based signal amplifier are developed. The conductive PPy nanowire (less than 10 nm in diameter) mesh architecture is uniformly dispersed within polymeric matrix via template-free in situ synthesis. Serum CRP levels are quantitatively analyzed through monitoring the conductance change caused by polymeric network shrinkage upon the aptamer-CRP binding. The limit of detection (LOD) of the polymeric sensor for human CRP sample can reach 7.85 × 10−19m. This CRP-specific biosensor and a commercial CRP enzyme-linked immunosorbent assay (ELISA) kit are used to perform side-by-side measurement of serum CRP in melanoma patients. The results indicate that this conductive polymeric senor is highly sensitive and selective in accurately discriminating melanoma patients from healthy controls using serum CRP as a biomarker, which is further validated by a commercial human CRP ELISA kit. Collectively, this novel ultrasensitive nanowire-based polymeric biosensor may hold promise in biomarker detection and diagnosis of cancer.

Original languageEnglish (US)
Article number1802482
JournalAdvanced Functional Materials
Volume28
Issue number31
DOIs
StatePublished - Aug 1 2018

Keywords

  • biomarkers
  • biosensors
  • melanoma
  • nanowire mesh
  • polypyrrole

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

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