A composite chitosan-reinforced scaffold fails to provide osteochondral regeneration

Alice Roffi, Elizaveta Kon, Francesco Perdisa, Milena Fini, Alessandro Di Martino, Annapaola Parrilli, Francesca Salamanna, Monica Sandri, Maria Sartori, Simone Sprio, Anna Tampieri, Maurilio Marcacci, Giuseppe Filardo

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Several biomaterials have recently been developed to address the challenge of osteochondral regeneration. Among these, chitosan holds promises both for cartilage and bone healing. The aim of this in vivo study was to evaluate the regeneration potential of a novel hybrid magnesium-doped hydroxyapatite (MgHA), collagen, chitosan-based scaffold,whichwas tested in a sheepmodel to ascertain its osteochondral regenerative potential, and in a rabbitmodel to further evaluate its ability to regenerate bone tissue. Macroscopic, microtomography, histology, histomorphometry, and immunohistochemical analysis were performed. In the sheepmodel, all analyses did not show significant differences compared to untreated defects (p > 0.05), with no evidence of cartilage and subchondral bone regeneration. In the rabbit model, this bone scaffold provided less ability to enhance tissue healing compared with a commercial bone scaffold. Moreover, persistence of scaffold material and absence of integration with connective tissue around the scaffolds were observed. These results raised some concerns about the osteochondral use of this chitosan composite scaffold, especially for the bone layer. Further studies are needed to explore the best formulation of chitosan-reinforced composites for osteochondral treatment.

Original languageEnglish (US)
Article number2227
JournalInternational journal of molecular sciences
Issue number9
StatePublished - May 1 2019


  • Bone
  • Cartilage
  • Chitosan
  • Failure
  • Osteochondral
  • Scaffold

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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