TY - JOUR
T1 - A comparative study on the hepatic in vitro metabolism of 4-androstene-3, 17-dione in the hagfish, Myxine glutinosa, the dogfish, Squalus acanthias, and the rainbow trout, Salmo gairdnerii
AU - Hansson, Tiiu
AU - Rafter, Joseph
AU - Gustafsson, Jan Åke
N1 - Funding Information:
This work was supported by grants from the Swedish Medical Research Council (No. 2819) and from Harald Jeanssons Stiftelse samt Harald och Greta Jeanssons Stiftelse. J. Rafter is grateful to Stif-telsen Bengt Lundqvists Minne for a fellowship.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1979/2
Y1 - 1979/2
N2 - The metabolism of 4-androstene-3, 17-dione (androstenedione) by liver microsomes from hagfish, Myxine glutinosa, dogfish, Squalus acanthias, and rainbow trout, Salmo gairdnerii, was studied. The following reduced metabolites were formed in the hagfish: testosterone, 3α-hydroxy-5α-androstan-17-one and 5α-androstane-3, 17-dione. In addition, 3α-hydroxy-4-androsten-17-one was formed by liver microsomes from dogfish and rainbow trout. While the patterns of reduced metabolites were very similar, there was a marked variation in the patterns of hydroxylated metabolites produced by the different species. Liver microsomes from hagfish gave rise to 6β-hydroxytestosterone and 6β-hydroxyandrostenedione. In addition to 6β-hydroxylation, 6α- and 16β-hydroxylations were observed in liver microsomes from dogfish. Liver microsomes from rainbow trout performed all the hydroxylations mentioned and were also capable of 1α-hydroxylation. The formation of hydroxylated metabolites was inhibited by CO indicating the participation of cytochrome P-450-dependent enzymes.
AB - The metabolism of 4-androstene-3, 17-dione (androstenedione) by liver microsomes from hagfish, Myxine glutinosa, dogfish, Squalus acanthias, and rainbow trout, Salmo gairdnerii, was studied. The following reduced metabolites were formed in the hagfish: testosterone, 3α-hydroxy-5α-androstan-17-one and 5α-androstane-3, 17-dione. In addition, 3α-hydroxy-4-androsten-17-one was formed by liver microsomes from dogfish and rainbow trout. While the patterns of reduced metabolites were very similar, there was a marked variation in the patterns of hydroxylated metabolites produced by the different species. Liver microsomes from hagfish gave rise to 6β-hydroxytestosterone and 6β-hydroxyandrostenedione. In addition to 6β-hydroxylation, 6α- and 16β-hydroxylations were observed in liver microsomes from dogfish. Liver microsomes from rainbow trout performed all the hydroxylations mentioned and were also capable of 1α-hydroxylation. The formation of hydroxylated metabolites was inhibited by CO indicating the participation of cytochrome P-450-dependent enzymes.
UR - http://www.scopus.com/inward/record.url?scp=0018763606&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018763606&partnerID=8YFLogxK
U2 - 10.1016/0016-6480(79)90112-6
DO - 10.1016/0016-6480(79)90112-6
M3 - Article
C2 - 447064
AN - SCOPUS:0018763606
SN - 0016-6480
VL - 37
SP - 240
EP - 245
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 2
ER -