A compact and low-cost solution for rapid frequency domain lifetime imaging

Lakhvir Singh, Linghao Hu, Alex J. Walsh

Research output: Contribution to conferencePaper

Abstract

A cost friendly and compact frequency domain fluorescent lifetime microscope (FD-FLIM), with the potential of capturing lifetimes with resolution of a few nanoseconds, has been theorized and designed using detection signal processing to encode the phase shift of fluorescence emission within the intensity captured via camera. Model simulations of the system parameters illustrate the calibration process and effectiveness of the technique. The results will facilitate the development of a novel FD-FLIM that uses simple optical components and a conventional camera for improved modularity, ergonomics, software-interface, precision control, automation, compactness, and cost-effectiveness over traditional lifetime microscopes.

Original languageEnglish (US)
StatePublished - 2024
EventMicroscopy Histopathology and Analytics, Microscopy 2024 - Part of Optica Biophotonics Congress: Biomedical Optics - Fort Lauderdale, United States
Duration: Apr 7 2024Apr 10 2024

Conference

ConferenceMicroscopy Histopathology and Analytics, Microscopy 2024 - Part of Optica Biophotonics Congress: Biomedical Optics
Country/TerritoryUnited States
CityFort Lauderdale
Period4/7/244/10/24

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Biomedical Engineering
  • Atomic and Molecular Physics, and Optics

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