A combined flat‐embedding, HRP histochemical method for correlative light and electron microscopic study of single neurons

L. D. Aldes, Timothy B. Boone

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

A simple and reproducible method is described for studying the morphology of the same neuron at the light and electron microscopic level. This method utilizes horseradish peroxidase histochemistry and a recently described flat-embedding procedure wherein thin, aldehyde-fixed sections are placed in resin between glass microscope slides pretreated with dimethyldichlorosilane. The significance of these combined methodologies for correlative light and electron microscopic studies of single neurons is discussed.

Original languageEnglish (US)
Pages (from-to)27-34
Number of pages8
JournalJournal of Neuroscience Research
Volume11
Issue number1
DOIs
StatePublished - Jan 1 1984

Keywords

  • HRP
  • dimethyldichlorosilane
  • flat‐embedding
  • light and electron microscopy

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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