A census of human soluble protein complexes

Pierre C. Havugimana, G. Traver Hart, Tamás Nepusz, Haixuan Yang, Andrei L. Turinsky, Zhihua Li, Peggy I. Wang, Daniel R. Boutz, Vincent Fong, Sadhna Phanse, Mohan Babu, Stephanie A. Craig, Pingzhao Hu, Cuihong Wan, James Vlasblom, Vaqaar Un Nisa Dar, Alexandr Bezginov, Gregory W. Clark, Gabriel C. Wu, Shoshana J. WodakElisabeth R.M. Tillier, Alberto Paccanaro, Edward M. Marcotte, Andrew Emili

Research output: Contribution to journalArticlepeer-review

Abstract

Cellular processes often depend on stable physical associations between proteins. Despite recent progress, knowledge of the composition of human protein complexes remains limited. To close this gap, we applied an integrative global proteomic profiling approach, based on chromatographic separation of cultured human cell extracts into more than one thousand biochemical fractions that were subsequently analyzed by quantitative tandem mass spectrometry, to systematically identify a network of 13,993 high-confidence physical interactions among 3,006 stably associated soluble human proteins. Most of the 622 putative protein complexes we report are linked to core biological processes and encompass both candidate disease genes and unannotated proteins to inform on mechanism. Strikingly, whereas larger multiprotein assemblies tend to be more extensively annotated and evolutionarily conserved, human protein complexes with five or fewer subunits are far more likely to be functionally unannotated or restricted to vertebrates, suggesting more recent functional innovations.

Original languageEnglish (US)
Pages (from-to)1068-1081
Number of pages14
JournalCell
Volume150
Issue number5
DOIs
StatePublished - Aug 31 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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