@article{91fc1321c38c46bd8f9890229039e9f0,
title = "A census of human soluble protein complexes",
abstract = "Cellular processes often depend on stable physical associations between proteins. Despite recent progress, knowledge of the composition of human protein complexes remains limited. To close this gap, we applied an integrative global proteomic profiling approach, based on chromatographic separation of cultured human cell extracts into more than one thousand biochemical fractions that were subsequently analyzed by quantitative tandem mass spectrometry, to systematically identify a network of 13,993 high-confidence physical interactions among 3,006 stably associated soluble human proteins. Most of the 622 putative protein complexes we report are linked to core biological processes and encompass both candidate disease genes and unannotated proteins to inform on mechanism. Strikingly, whereas larger multiprotein assemblies tend to be more extensively annotated and evolutionarily conserved, human protein complexes with five or fewer subunits are far more likely to be functionally unannotated or restricted to vertebrates, suggesting more recent functional innovations.",
author = "Havugimana, {Pierre C.} and Hart, {G. Traver} and Tam{\'a}s Nepusz and Haixuan Yang and Turinsky, {Andrei L.} and Zhihua Li and Wang, {Peggy I.} and Boutz, {Daniel R.} and Vincent Fong and Sadhna Phanse and Mohan Babu and Craig, {Stephanie A.} and Pingzhao Hu and Cuihong Wan and James Vlasblom and Dar, {Vaqaar Un Nisa} and Alexandr Bezginov and Clark, {Gregory W.} and Wu, {Gabriel C.} and Wodak, {Shoshana J.} and Tillier, {Elisabeth R.M.} and Alberto Paccanaro and Marcotte, {Edward M.} and Andrew Emili",
note = "Funding Information: We thank R. Isserlin, Z. Ni, H. Guo, D. Merico and A. Alpert for technical assistance and J. Parkinson, G. Bader, A. Wilde, and J. Greenblatt for critical suggestions. P.C.H. was a recipient of a University of Toronto Open Fellowship, T.N. was supported by the Newton International Fellowship Scheme of the Royal Society, A.E. is an Ontario Research Chair, and S.J.W. is a Canada Research Chair Tier 1. This work was supported by grants from the Biotechnology and Biological Sciences Research Council (BB/F00964X/1 and BB/K004131/1) and the Royal Society (NF080750) to A.P., the Canada Institutes of Health Research (MOP 82940) and the SickKids Foundation to S.J.W., the National Institutes of Health, National Science Foundation, Cancer Prevention Research Institute of Texas, and Welch (F1515) and Packard Foundations to E.M.M., and the Ontario Ministry of Research and Innovation to A.E. ",
year = "2012",
month = aug,
day = "31",
doi = "10.1016/j.cell.2012.08.011",
language = "English (US)",
volume = "150",
pages = "1068--1081",
journal = "Cell",
issn = "0092-8674",
publisher = "Elsevier B.V.",
number = "5",
}