A bioluminescent transgenic mouse model: Real-time in vivo imaging of antioxidant EC-SOD gene expression and regulation by interferon gamma

Igor N. Zelko, Marcus W. Stepp, Rodney J. Folz

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Extracellular superoxide dismutase (EC-SOD) is the main antioxidant enzyme in the extracellular matrix. We developed transgenic mice to analyze the EC-SOD promoter activity in vivo in real time and to identify the important cis-elements flanking the 5' region of the murine EC-SOD gene. Using this model, we demonstrated that luciferase reporter activity correlates closely with endogenous EC-SOD expression, although several interesting differences were also observed. Specifically, luciferase activity was detected at the highest levels in testes, aorta and perirenal fat. Reporter expression was regulated by interferon gamma, a finding that is in agreement with published endogenous EC-SOD gene expression studies. Thus, the 5'-flanking region of mouse EC-SOD gene is responsible, at least in part, for cell specific and inducible expression.

Original languageEnglish (US)
Pages (from-to)75-82
Number of pages8
JournalGene
Volume530
Issue number1
DOIs
StatePublished - Nov 1 2013

Keywords

  • Expression
  • Interferon gamma
  • Oxidative stress
  • Superoxide dismutase
  • Transcription
  • Transgenic mice

ASJC Scopus subject areas

  • Genetics

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