A Bidirectional Single-Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration

Ning Shao; Yufu Zhou; Jun Yao; Pengchao Zhang; Yanni Song; Kai Zhang; Xin Han; Bin Wang; Xuewu Liu

doi:10.1002/advs.202204544

A Bidirectional Single-Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration

Ning Shao, Yufu Zhou, Jun Yao, Pengchao Zhang, Yanni Song, Kai Zhang, Xin Han, Bin Wang, Xuewu Liu

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Dendritic cell (DC) migration is a fundamental step during execution of its adaptive immunity functions. Studying DC migration characteristics is critical for development of DC-dependent allergy treatments, vaccines, and cancer immunotherapies. Here, a microfluidics-based single-cell migration platform is described that enables high-throughput and precise bidirectional cell migration assays. It also allows selective retrieval of cell subpopulations that have different migratory potentials. Using this microfluidic platform, DC migration is investigated in response to different chemoattractants and inhibitors, quantitatively describe DC migration patterns and retrieve DC subpopulations of different migratory potentials for differential gene expression analysis. This platform opens an avenue for precise characterization of cell migration and potential discovery of therapeutic modulators.

Original language	English (US)
Article number	2204544
Pages (from-to)	e2204544
Journal	Advanced Science
Volume	10
Issue number	8
DOIs	https://doi.org/10.1002/advs.202204544
State	Published - Mar 15 2023

Keywords

RNA-seq
cell retrieval
dendritic cell
microfluidics
single cell migration

ASJC Scopus subject areas

Medicine (miscellaneous)
Chemical Engineering(all)
Materials Science(all)
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Engineering(all)
Physics and Astronomy(all)

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10.1002/advs.202204544

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title = "A Bidirectional Single-Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration",

abstract = "Dendritic cell (DC) migration is a fundamental step during execution of its adaptive immunity functions. Studying DC migration characteristics is critical for development of DC-dependent allergy treatments, vaccines, and cancer immunotherapies. Here, a microfluidics-based single-cell migration platform is described that enables high-throughput and precise bidirectional cell migration assays. It also allows selective retrieval of cell subpopulations that have different migratory potentials. Using this microfluidic platform, DC migration is investigated in response to different chemoattractants and inhibitors, quantitatively describe DC migration patterns and retrieve DC subpopulations of different migratory potentials for differential gene expression analysis. This platform opens an avenue for precise characterization of cell migration and potential discovery of therapeutic modulators.",

keywords = "RNA-seq, cell retrieval, dendritic cell, microfluidics, single cell migration",

author = "Ning Shao and Yufu Zhou and Jun Yao and Pengchao Zhang and Yanni Song and Kai Zhang and Xin Han and Bin Wang and Xuewu Liu",

note = "Funding Information: N.S. and Y.Z. contributed equally to this work. The authors are grateful for funding support from the original NIH 1R21CA191179‐01A1 and its supplement, and NIH U01CA244107. Publisher Copyright: {\textcopyright} 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.",

year = "2023",

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doi = "10.1002/advs.202204544",

language = "English (US)",

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T1 - A Bidirectional Single-Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration

AU - Shao, Ning

AU - Zhou, Yufu

AU - Yao, Jun

AU - Zhang, Pengchao

AU - Song, Yanni

AU - Zhang, Kai

AU - Han, Xin

AU - Wang, Bin

AU - Liu, Xuewu

N1 - Funding Information: N.S. and Y.Z. contributed equally to this work. The authors are grateful for funding support from the original NIH 1R21CA191179‐01A1 and its supplement, and NIH U01CA244107. Publisher Copyright: © 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.

PY - 2023/3/15

Y1 - 2023/3/15

N2 - Dendritic cell (DC) migration is a fundamental step during execution of its adaptive immunity functions. Studying DC migration characteristics is critical for development of DC-dependent allergy treatments, vaccines, and cancer immunotherapies. Here, a microfluidics-based single-cell migration platform is described that enables high-throughput and precise bidirectional cell migration assays. It also allows selective retrieval of cell subpopulations that have different migratory potentials. Using this microfluidic platform, DC migration is investigated in response to different chemoattractants and inhibitors, quantitatively describe DC migration patterns and retrieve DC subpopulations of different migratory potentials for differential gene expression analysis. This platform opens an avenue for precise characterization of cell migration and potential discovery of therapeutic modulators.

AB - Dendritic cell (DC) migration is a fundamental step during execution of its adaptive immunity functions. Studying DC migration characteristics is critical for development of DC-dependent allergy treatments, vaccines, and cancer immunotherapies. Here, a microfluidics-based single-cell migration platform is described that enables high-throughput and precise bidirectional cell migration assays. It also allows selective retrieval of cell subpopulations that have different migratory potentials. Using this microfluidic platform, DC migration is investigated in response to different chemoattractants and inhibitors, quantitatively describe DC migration patterns and retrieve DC subpopulations of different migratory potentials for differential gene expression analysis. This platform opens an avenue for precise characterization of cell migration and potential discovery of therapeutic modulators.

KW - RNA-seq

KW - cell retrieval

KW - dendritic cell

KW - microfluidics

KW - single cell migration

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A Bidirectional Single-Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration

Abstract

Keywords

ASJC Scopus subject areas

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