TY - JOUR
T1 - 4-chlorobiphenyl metabolism
T2 - The effects of chemical inducers
AU - Crawford, Andrew
AU - Safe, Stephen
N1 - Funding Information:
Acknowledgements--This study was supported in part by the Research Programs Directorate. Health and Welfare Canada, (606-1444-X1, the National Cancer Institute (U.S.A.), DHEW, Grant Number 1 B01 CA21814-01 and the National Research Council of Canada.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1979
Y1 - 1979
N2 - 1. 1. The total metabolism of 4-chlorobiphenyl by rat hepatic microsomes to give lipophilic phenolic products, trichloracetic acid-soluble conjugates and macromolecular-substrate adducts has been determined. 2. 2. The metabolism of this substrate by cytochrome P-450, cytochrome P-448 and mixed cytochrome P-450/P-448 induced microsomal enzymes yielded a single major phenolic product, 4′-chloro-4-biphenylol. 3. 3. Microsomal enzymes from animals pretreated with cytochrome P-448 and mixed cytochrome P-450/P-448 chemical inducers enhanced the metabolic activity whereas the activity with cytochrome P-450 induced enzymes was similar to the non-induced enzyme system. 4. 4. The unusual metabolic activity of this substrate indicated the important directive effects of the chloro substituent and suggested the potential utility of this hydroxylase assay in evaluating the effects of microsomal enzyme inducers.
AB - 1. 1. The total metabolism of 4-chlorobiphenyl by rat hepatic microsomes to give lipophilic phenolic products, trichloracetic acid-soluble conjugates and macromolecular-substrate adducts has been determined. 2. 2. The metabolism of this substrate by cytochrome P-450, cytochrome P-448 and mixed cytochrome P-450/P-448 induced microsomal enzymes yielded a single major phenolic product, 4′-chloro-4-biphenylol. 3. 3. Microsomal enzymes from animals pretreated with cytochrome P-448 and mixed cytochrome P-450/P-448 chemical inducers enhanced the metabolic activity whereas the activity with cytochrome P-450 induced enzymes was similar to the non-induced enzyme system. 4. 4. The unusual metabolic activity of this substrate indicated the important directive effects of the chloro substituent and suggested the potential utility of this hydroxylase assay in evaluating the effects of microsomal enzyme inducers.
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U2 - 10.1016/0306-3623(79)90094-6
DO - 10.1016/0306-3623(79)90094-6
M3 - Article
C2 - 112002
AN - SCOPUS:0018749299
SN - 0306-3623
VL - 10
SP - 227
EP - 231
JO - General Pharmacology
JF - General Pharmacology
IS - 3
ER -