TY - JOUR
T1 - 3D bioprinted mesenchymal stem cell laden scaffold enhances subcutaneous vascularization for delivery of cell therapy
AU - Bo, Tommaso
AU - Pascucci, Elia
AU - Capuani, Simone
AU - Campa-Carranza, Jocelyn Nikita
AU - Franco, Letizia
AU - Farina, Marco
AU - Secco, Jacopo
AU - Becchi, Sara
AU - Cavazzana, Rosanna
AU - Joubert, Ashley L.
AU - Hernandez, Nathanael
AU - Chua, Corrine Ying Xuan
AU - Grattoni, Alessandro
N1 - © 2024. The Author(s).
PY - 2024/6/18
Y1 - 2024/6/18
N2 - Subcutaneous delivery of cell therapy is an appealing minimally-invasive strategy for the treatment of various diseases. However, the subdermal site is poorly vascularized making it inadequate for supporting engraftment, viability, and function of exogenous cells. In this study, we developed a 3D bioprinted scaffold composed of alginate/gelatin (Alg/Gel) embedded with mesenchymal stem cells (MSCs) to enhance vascularization and tissue ingrowth in a subcutaneous microenvironment. We identified bio-ink crosslinking conditions that optimally recapitulated the mechanical properties of subcutaneous tissue. We achieved controlled degradation of the Alg/Gel scaffold synchronous with host tissue ingrowth and remodeling. Further, in a rat model, the Alg/Gel scaffold was superior to MSC-embedded Pluronic hydrogel in supporting tissue development and vascularization of a subcutaneous site. While the scaffold alone promoted vascular tissue formation, the inclusion of MSCs in the bio-ink further enhanced angiogenesis. Our findings highlight the use of simple cell-laden degradable bioprinted structures to generate a supportive microenvironment for cell delivery.
AB - Subcutaneous delivery of cell therapy is an appealing minimally-invasive strategy for the treatment of various diseases. However, the subdermal site is poorly vascularized making it inadequate for supporting engraftment, viability, and function of exogenous cells. In this study, we developed a 3D bioprinted scaffold composed of alginate/gelatin (Alg/Gel) embedded with mesenchymal stem cells (MSCs) to enhance vascularization and tissue ingrowth in a subcutaneous microenvironment. We identified bio-ink crosslinking conditions that optimally recapitulated the mechanical properties of subcutaneous tissue. We achieved controlled degradation of the Alg/Gel scaffold synchronous with host tissue ingrowth and remodeling. Further, in a rat model, the Alg/Gel scaffold was superior to MSC-embedded Pluronic hydrogel in supporting tissue development and vascularization of a subcutaneous site. While the scaffold alone promoted vascular tissue formation, the inclusion of MSCs in the bio-ink further enhanced angiogenesis. Our findings highlight the use of simple cell-laden degradable bioprinted structures to generate a supportive microenvironment for cell delivery.
KW - 3D bioprinting
KW - Cell therapy
KW - Mesenchymal stem cells
KW - Regenerative medicine
KW - Tissue engineering
KW - Vascularized scaffold
KW - Bioprinting
KW - Hydrogels/chemistry
KW - Rats
KW - Alginates/chemistry
KW - Cell- and Tissue-Based Therapy
KW - Rats, Sprague-Dawley
KW - Mesenchymal Stem Cells/cytology
KW - Animals
KW - Tissue Scaffolds/chemistry
KW - Subcutaneous Tissue
KW - Printing, Three-Dimensional
KW - Neovascularization, Physiologic
KW - Mesenchymal Stem Cell Transplantation
KW - Gelatin/chemistry
UR - http://www.scopus.com/inward/record.url?scp=85196106360&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85196106360&partnerID=8YFLogxK
U2 - 10.1007/s10544-024-00713-2
DO - 10.1007/s10544-024-00713-2
M3 - Article
C2 - 38888669
AN - SCOPUS:85196106360
SN - 1387-2176
VL - 26
SP - 29
JO - Biomedical Microdevices
JF - Biomedical Microdevices
IS - 3
M1 - 29
ER -