3-methylcholanthrene induces differential recruitment of aryl hydrocarbon receptor to human promoters

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The paper by Pansoy and coworkers investigates the effects of the aryl hydrocarbon receptor (AHR) ligand 3-methylcholanthrene (3MC) on recruitment of the AHR complex to human promoters in T47D breast cancer cells. The results are particularly important because they can be compared with a prior study using the potent AHR ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the same cell line. The chromatin immunoprecipitation and promoter-focused microarrays (ChIP-chip) demonstrated that after treatment of T47D cells with 1μM 3MC, there were 241 AHR-3MC bound regions and many of these contained AHR-responsive elements. However, they also observed interactions with regions that do not contain these responsive elements, and subsequent analysis of selected target genes show that 3MC-dependent AHR binding did not necessarily predict Ah-responsiveness because induction, repression, and no effects were observed. A prior study with TCDD demonstrated that both 3MC and TCDD induced AHR binding to 127 common regions; however, there were significant differences in ligand (3MC vs. TCDD)-dependent AHR bound regions. The results illustrate the complexity of AHR signaling and also demonstrate that compared with TCDD as a reference ligand, 3MC is a selective AHR modulator.

Original languageEnglish (US)
Pages (from-to)1-3
Number of pages3
JournalToxicological Sciences
Volume117
Issue number1
DOIs
StatePublished - Jul 22 2010
Externally publishedYes

Keywords

  • AhR
  • AhR binding sites
  • ChIP
  • Ligand differences

ASJC Scopus subject areas

  • Toxicology

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