3-D axon structure extraction and analysis in confocal fluorescence microscopy images

Yong Zhang, Xiaobo Zhou, Ju Lu, Jeff Lichtman, Donald Adjeroh, Stephen TC Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

In this paper, we propose a highly automated 3-D centerline extraction tool to assist 3-D microscopy image analysis to extract morphological features of the neuronal structures. For this project, the most difficult part is that, some axons are overlapping to each other that the boundaries distinguishing them are hardly visible. Our approach combines 3-D Dynamic Programming (DP) technique and marker controlled watershed algorithm to solve this problem. The approach consists of tracking and updating along the navigation directions of multiple axons simultaneously. The experimental results show that, the proposed method can rapidly and accurately extract multiple axon centerlines and can handle complicated axon structures such as cross-over sections and overlapping objects.

Original languageEnglish (US)
Title of host publication2007 IEEE/NIH Life Science Systems and Applications Workshop, LISA
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages241-244
Number of pages4
ISBN (Print)9781424418138
DOIs
StatePublished - 2007
Event2007 IEEE/NIH Life Science Systems and Applications Workshop, LISA - Bethesda, MD, United States
Duration: Nov 8 2007Nov 9 2007

Publication series

Name2007 IEEE/NIH Life Science Systems and Applications Workshop, LISA

Other

Other2007 IEEE/NIH Life Science Systems and Applications Workshop, LISA
CountryUnited States
CityBethesda, MD
Period11/8/0711/9/07

ASJC Scopus subject areas

  • Computer Science Applications
  • Information Systems

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