3β-Hydroxy-Δ5-C19- and C21-steroid oxidoreductase activity in rat liver

Ingemar Björkhem; Kurt Einarsson; Jan Åke Gustafsson

doi:10.1016/S0039-128X(72)80015-1

3β-Hydroxy-Δ⁵-C₁₉- and C₂₁-steroid oxidoreductase activity in rat liver

Ingemar Björkhem, Kurt Einarsson, Jan Åke Gustafsson

Houston Methodist

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The presence of small amounts of 3β-hydroxy-Δ⁵-C₁₉- and C₂₁-steroid oxidoreductase in the microsomal fraction of rat liver is shown. NAD was the preferred cofactor. K_m for the oxidation of dehydroepiandrosterone (3β-hydroxy-5-androsten-17-one) into androstenedione (4-androstene-3,17-dione) was 3 × 10^-6 M. In similarity to the adrenal and gonadal 3β-hydroxy-Δ⁵-C₁₉-steroid oxidoreductase, but in contrast to the hepatic 3β-hydroxy-Δ⁵-C₂₇-steroid oxidoreductase involved in the biosynthesis of bile acids, the hepatic 3β-hydroxy-Δ⁵-C₁₉-steroid oxidoreductase was inhibited by the 3β-hydroxy-Δ⁵-steroid oxidoreductase inhibitor, 2α-cyano-4,4,17-trimethyl-17β-hydroxy-5-androsten-3-one, and the activity was greatly reduced with microsomes from immature rats.

Original language	English (US)
Pages (from-to)	471-476
Number of pages	6
Journal	Steroids
Volume	19
Issue number	4
DOIs	https://doi.org/10.1016/S0039-128X(72)80015-1
State	Published - Apr 1972

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology
Pharmacology
Clinical Biochemistry
Organic Chemistry

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10.1016/S0039-128X(72)80015-1

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@article{0a579a180cc641db85085d5776ea30d5,

title = "3β-Hydroxy-Δ5-C19- and C21-steroid oxidoreductase activity in rat liver",

abstract = "The presence of small amounts of 3β-hydroxy-Δ5-C19- and C21-steroid oxidoreductase in the microsomal fraction of rat liver is shown. NAD was the preferred cofactor. Km for the oxidation of dehydroepiandrosterone (3β-hydroxy-5-androsten-17-one) into androstenedione (4-androstene-3,17-dione) was 3 × 10-6 M. In similarity to the adrenal and gonadal 3β-hydroxy-Δ5-C19-steroid oxidoreductase, but in contrast to the hepatic 3β-hydroxy-Δ5-C27-steroid oxidoreductase involved in the biosynthesis of bile acids, the hepatic 3β-hydroxy-Δ5-C19-steroid oxidoreductase was inhibited by the 3β-hydroxy-Δ5-steroid oxidoreductase inhibitor, 2α-cyano-4,4,17-trimethyl-17β-hydroxy-5-androsten-3-one, and the activity was greatly reduced with microsomes from immature rats.",

author = "Ingemar Bj{\"o}rkhem and Kurt Einarsson and Gustafsson, \{Jan {\AA}ke\}",

note = "Funding Information: The skilful technical assistance of Miss Yvonne Jacob is gratefully acknowledged. This work was supported by grants from the Swedish Medical Research Council (Projects 13X-218 and 13X-28i9). References Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1972",

month = apr,

doi = "10.1016/S0039-128X(72)80015-1",

language = "English (US)",

volume = "19",

pages = "471--476",

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AU - Björkhem, Ingemar

AU - Einarsson, Kurt

AU - Gustafsson, Jan Åke

N1 - Funding Information: The skilful technical assistance of Miss Yvonne Jacob is gratefully acknowledged. This work was supported by grants from the Swedish Medical Research Council (Projects 13X-218 and 13X-28i9). References Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1972/4

Y1 - 1972/4

N2 - The presence of small amounts of 3β-hydroxy-Δ5-C19- and C21-steroid oxidoreductase in the microsomal fraction of rat liver is shown. NAD was the preferred cofactor. Km for the oxidation of dehydroepiandrosterone (3β-hydroxy-5-androsten-17-one) into androstenedione (4-androstene-3,17-dione) was 3 × 10-6 M. In similarity to the adrenal and gonadal 3β-hydroxy-Δ5-C19-steroid oxidoreductase, but in contrast to the hepatic 3β-hydroxy-Δ5-C27-steroid oxidoreductase involved in the biosynthesis of bile acids, the hepatic 3β-hydroxy-Δ5-C19-steroid oxidoreductase was inhibited by the 3β-hydroxy-Δ5-steroid oxidoreductase inhibitor, 2α-cyano-4,4,17-trimethyl-17β-hydroxy-5-androsten-3-one, and the activity was greatly reduced with microsomes from immature rats.

AB - The presence of small amounts of 3β-hydroxy-Δ5-C19- and C21-steroid oxidoreductase in the microsomal fraction of rat liver is shown. NAD was the preferred cofactor. Km for the oxidation of dehydroepiandrosterone (3β-hydroxy-5-androsten-17-one) into androstenedione (4-androstene-3,17-dione) was 3 × 10-6 M. In similarity to the adrenal and gonadal 3β-hydroxy-Δ5-C19-steroid oxidoreductase, but in contrast to the hepatic 3β-hydroxy-Δ5-C27-steroid oxidoreductase involved in the biosynthesis of bile acids, the hepatic 3β-hydroxy-Δ5-C19-steroid oxidoreductase was inhibited by the 3β-hydroxy-Δ5-steroid oxidoreductase inhibitor, 2α-cyano-4,4,17-trimethyl-17β-hydroxy-5-androsten-3-one, and the activity was greatly reduced with microsomes from immature rats.

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ER -

3β-Hydroxy-Δ⁵-C₁₉- and C₂₁-steroid oxidoreductase activity in rat liver

Abstract

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