TY - JOUR
T1 - 3β-Hydroxy-Δ5-C19- and C21-steroid oxidoreductase activity in rat liver
AU - Björkhem, Ingemar
AU - Einarsson, Kurt
AU - Gustafsson, Jan Åke
N1 - Funding Information:
The skilful technical assistance of Miss Yvonne Jacob is gratefully acknowledged. This work was supported by grants from the Swedish Medical Research Council (Projects 13X-218 and 13X-28i9). References
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1972/4
Y1 - 1972/4
N2 - The presence of small amounts of 3β-hydroxy-Δ5-C19- and C21-steroid oxidoreductase in the microsomal fraction of rat liver is shown. NAD was the preferred cofactor. Km for the oxidation of dehydroepiandrosterone (3β-hydroxy-5-androsten-17-one) into androstenedione (4-androstene-3,17-dione) was 3 × 10-6 M. In similarity to the adrenal and gonadal 3β-hydroxy-Δ5-C19-steroid oxidoreductase, but in contrast to the hepatic 3β-hydroxy-Δ5-C27-steroid oxidoreductase involved in the biosynthesis of bile acids, the hepatic 3β-hydroxy-Δ5-C19-steroid oxidoreductase was inhibited by the 3β-hydroxy-Δ5-steroid oxidoreductase inhibitor, 2α-cyano-4,4,17-trimethyl-17β-hydroxy-5-androsten-3-one, and the activity was greatly reduced with microsomes from immature rats.
AB - The presence of small amounts of 3β-hydroxy-Δ5-C19- and C21-steroid oxidoreductase in the microsomal fraction of rat liver is shown. NAD was the preferred cofactor. Km for the oxidation of dehydroepiandrosterone (3β-hydroxy-5-androsten-17-one) into androstenedione (4-androstene-3,17-dione) was 3 × 10-6 M. In similarity to the adrenal and gonadal 3β-hydroxy-Δ5-C19-steroid oxidoreductase, but in contrast to the hepatic 3β-hydroxy-Δ5-C27-steroid oxidoreductase involved in the biosynthesis of bile acids, the hepatic 3β-hydroxy-Δ5-C19-steroid oxidoreductase was inhibited by the 3β-hydroxy-Δ5-steroid oxidoreductase inhibitor, 2α-cyano-4,4,17-trimethyl-17β-hydroxy-5-androsten-3-one, and the activity was greatly reduced with microsomes from immature rats.
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U2 - 10.1016/S0039-128X(72)80015-1
DO - 10.1016/S0039-128X(72)80015-1
M3 - Article
C2 - 4337647
AN - SCOPUS:0015323449
VL - 19
SP - 471
EP - 476
JO - Steroids
JF - Steroids
SN - 0039-128X
IS - 4
ER -