3β-Hydroxy-Δ5-C19- and C21-steroid oxidoreductase activity in rat liver

Ingemar Björkhem, Kurt Einarsson, Jan Åke Gustafsson

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The presence of small amounts of 3β-hydroxy-Δ5-C19- and C21-steroid oxidoreductase in the microsomal fraction of rat liver is shown. NAD was the preferred cofactor. Km for the oxidation of dehydroepiandrosterone (3β-hydroxy-5-androsten-17-one) into androstenedione (4-androstene-3,17-dione) was 3 × 10-6 M. In similarity to the adrenal and gonadal 3β-hydroxy-Δ5-C19-steroid oxidoreductase, but in contrast to the hepatic 3β-hydroxy-Δ5-C27-steroid oxidoreductase involved in the biosynthesis of bile acids, the hepatic 3β-hydroxy-Δ5-C19-steroid oxidoreductase was inhibited by the 3β-hydroxy-Δ5-steroid oxidoreductase inhibitor, 2α-cyano-4,4,17-trimethyl-17β-hydroxy-5-androsten-3-one, and the activity was greatly reduced with microsomes from immature rats.

Original languageEnglish (US)
Pages (from-to)471-476
Number of pages6
JournalSteroids
Volume19
Issue number4
DOIs
StatePublished - Apr 1972

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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