17β-Estradiol-mediated growth inhibition of MDA-MB-468 cells stably transfected with the estrogen receptor: Cell cycle effects

Weili Wang, Roger Smith, Robert Burghardt, Stephen H. Safe

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Estrogen receptor (ER)-negative MDA-MB-468 human breast cancer cells were stably transected with wild-type human ER and utilized as a model for investigating estrogen- and aryl hydrocarbon (Ah)-responsiveness. Treatment of the stably transfected cells with 10 nM 17β-estradiol (E2) resulted in a significant inhibition ( > 64%) of cell proliferation and DNA synthesis, which was blocked by 10-7 M ICI 182 780. Analysis by flow cytometry indicated that treatment with E2 increased the percentage of cells in G0/G1 (from 68.8 to 89.4) and decreased cells in S (from 18.4 to 3.4) and G2/M (from 12.8 to 7.2) phases of the cell cycle. The effects of E2 on the major cyclins, cyclin-dependent kinases and cyclin-dependent kinase inhibitors, retinoblastoma protein (RB), E2F-1, and cyclin-dependent kinase activities were also investigated in the stably transfected MDA-MB-468 cells. The results demonstrated that the growth inhibitory effects of 10-8 M E2 in ER stably transfected MDA-MB-468 cells were associated with modulation of several factors required for cell cycle progression and DNA synthesis, including significant induction of the cyclin-dependent kinase inhibitor p21(cip-1) ( > 4-fold increase after 12 h) and decreased E2Fl and PCNA protein levels. These results show that the growth-inhibitory effects of E2 in the stably transfected cells were due to multiple factors which result in growth arrest in G0/G1 and inhibition of DNA synthesis.

Original languageEnglish (US)
Pages (from-to)49-62
Number of pages14
JournalMolecular and cellular endocrinology
Volume133
Issue number1
DOIs
StatePublished - Sep 30 1997

Keywords

  • Estradiol
  • Growth inhibition
  • MDA-MB-468 cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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