16S rRNA beacons for bacterial monitoring during human space missions

Maia Larios-Sanz, Katerina D. Kourentzi, David Warmflash, Jeffrey Jones, Duane L. Pierson, Richard C. Willson, George E. Fox

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Microorganisms are unavoidable in space environments and their presence has, at times, been a source of problems. Concerns about disease during human space missions are particularly important considering the significant changes the immune system incurs during spaceflight and the history of microbial contamination aboard the Mir space station. Additionally, these contaminants may have adverse effects on instrumentation and life-support systems. A sensitive, highly specific system to detect, characterize, and monitor these microbial populations is essential. Herein we describe a monitoring approach that uses 16S rRNA targeted molecular beacons to successfully detect several specific bacterial groupings. This methodology will greatly simplify in-flight monitoring by minimizing sample handling and processing. We also address and provide solutions to target accessibility problems encountered in hybridizations that target 16S rRNA.

Original languageEnglish (US)
JournalAviation Space and Environmental Medicine
Volume78
Issue number4 II
StatePublished - Apr 1 2007

Keywords

  • 16s rRNA
  • Bacteria
  • Monitoring
  • Spacecraft

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Pollution
  • Medicine(all)

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