TY - JOUR
T1 - 16-Channel Flexible System to Measure Electrophysiological Properties of Bioengineered Hearts
AU - Salazar, Betsy H.
AU - Hoffman, Kristopher A.
AU - Reddy, Anilkumar K.
AU - Madala, Sridhar
AU - Birla, Ravi K.
N1 - Funding Information:
The researchers would like to acknowledge NIH for provision of funding for this research (Grant Number: R01-EB011516). We would also like to thank the Department of Biomedical Engineering and the Cullen College of Engineering at University of Houston for further financial support. The Institutional Animal Care and Use Committee (IACUC) at the University of Houston, approved all animal protocols in accordance with the ‘‘Guide for the Care and Use of Laboratory Animals’’ (NIH publication 86-23, 1986). No human studies were carried out by the authors for this article.
Funding Information:
Dr. Ravi Birla has received research Grant(s) Number R01-EB011516, from the National Institute of Health. Betsy Salazar, Kristopher Hoffman, Dr. Anilkumar Reddy, and Dr. Sridhar Madala declare that they have no conflict of interest.
Funding Information:
The researchers would like to acknowledge NIH for provision of funding for this research (Grant Number: R01-EB011516). We would also like to thank the Department of Biomedical Engineering and the Cullen College of Engineering at University of Houston for further financial support. The Institutional Animal Care and Use Committee (IACUC) at the University of Houston, approved all animal protocols in accordance with the “Guide for the Care and Use of Laboratory Animals” (NIH publication 86-23, 1986). No human studies were carried out by the authors for this article. Dr. Ravi Birla has received research Grant(s) Number R01-EB011516, from the National Institute of Health. Betsy Salazar, Kristopher Hoffman, Dr. Anilkumar Reddy, and Dr. Sridhar Madala declare that they have no conflict of interest.
Publisher Copyright:
© 2017, Biomedical Engineering Society.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2018/3/1
Y1 - 2018/3/1
N2 - As tissue engineering continues to mature, it is necessary to develop new technologies that bring insight into current paradigms and guide improvements for future experiments. To this end, we have developed a system to characterize our bioartificial heart model and compare them to functional native structures. In the present study, the hearts of adult Sprague–Dawley were decellularized resulting in a natural three-dimensional cardiac scaffold. Neonatal rat primary cardiac cells were then cultured within a complex 3D fibrin gel, forming a 3-dimensional cardiac construct, which was sutured to the acellular scaffold and suspended in media for 24–48 h. The resulting bioartificial hearts (BAHs) were then affixed with 16 electrodes, in different configurations to evaluate not only the electrocardiographic characteristics of the cultured tissues, but to also test the system’s consistency. Histological evaluation showed cellularization and cardiac tissue formation. The BAHs and native hearts were then evaluated with our 16-channel flexible system to acquire the metrics associated with their respective electrophysiological properties. Time delays between the native signals were in the range of 0–95 ms. As well, color maps revealed a trend in impulse propagation throughout the native hearts. After evaluation of the normal rat QRS complex we found the average amplitude of the R-wave to be 5351.48 ± 44.92 μV and the average QRS duration was found to be 10.61 ± 0.18 ms. In contrast, BAHs exhibited more erratic and non-uniform activity that garnered no appreciable quantification. The data collected in this study proves our system’s efficacy for EKG data procurement.
AB - As tissue engineering continues to mature, it is necessary to develop new technologies that bring insight into current paradigms and guide improvements for future experiments. To this end, we have developed a system to characterize our bioartificial heart model and compare them to functional native structures. In the present study, the hearts of adult Sprague–Dawley were decellularized resulting in a natural three-dimensional cardiac scaffold. Neonatal rat primary cardiac cells were then cultured within a complex 3D fibrin gel, forming a 3-dimensional cardiac construct, which was sutured to the acellular scaffold and suspended in media for 24–48 h. The resulting bioartificial hearts (BAHs) were then affixed with 16 electrodes, in different configurations to evaluate not only the electrocardiographic characteristics of the cultured tissues, but to also test the system’s consistency. Histological evaluation showed cellularization and cardiac tissue formation. The BAHs and native hearts were then evaluated with our 16-channel flexible system to acquire the metrics associated with their respective electrophysiological properties. Time delays between the native signals were in the range of 0–95 ms. As well, color maps revealed a trend in impulse propagation throughout the native hearts. After evaluation of the normal rat QRS complex we found the average amplitude of the R-wave to be 5351.48 ± 44.92 μV and the average QRS duration was found to be 10.61 ± 0.18 ms. In contrast, BAHs exhibited more erratic and non-uniform activity that garnered no appreciable quantification. The data collected in this study proves our system’s efficacy for EKG data procurement.
KW - Bioengineered hearts
KW - Cardiac constructs
KW - Cell culture
KW - Electrical impulse propagation maps
KW - Heart
KW - Heart electrophysiology
KW - Tissue engineering
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U2 - 10.1007/s13239-017-0336-8
DO - 10.1007/s13239-017-0336-8
M3 - Article
C2 - 29150791
AN - SCOPUS:85041443257
SN - 1869-408X
VL - 9
SP - 94
EP - 104
JO - Cardiovascular Engineering and Technology
JF - Cardiovascular Engineering and Technology
IS - 1
ER -