β-Catenin nuclear localization is associated with grade in ovarian serous carcinoma

Christine M. Lee, Hyun Shvartsman, Michael T. Deavers, Shao Chun Wang, Weiya Xia, Rosemarie Schmandt, Diane C. Bodurka, E. Neely Atkinson, Anais Malpica, David M. Gershenson, Mien Chie Hung, Karen H. Lu

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Objectives. β-Catenin has been previously associated with oncogenic activity in human cancers. We evaluated whether β-catenin also plays a role in papillary serous ovarian neoplasms. Methods. Immunohistochemistry for β-catenin was performed on the primary ovarian serous neoplasms of 105 women. Of these, 10 were low malignant potential (LMP) serous tumors, and 95 were serous cancers. Nuclear β-catenin staining was correlated with grade of tumor and median survival. OVCAR-3, OVCA-420, OVCA-432, and MDAH-277-10c were evaluated for β-catenin localization and transfected with a T-cell factor (TCF) responsive reporter to evaluate β-catenin transcriptional activity. Results. Of 105 serous tumors, 13 (12.3%) demonstrated β-catenin nuclear staining. Eleven of 48 high-grade serous carcinomas (23.0%) demonstrated nuclear staining compared with 1 low-grade serous carcinoma (2.1%) (P = 0.006). One LMP tumor had nuclear staining. β-Catenin nuclear localization was undetectable in the cell lines tested. Furthermore, transient transfection of the cell lines with a TCF-responsive reporter did not demonstrate significant constitutive transcriptional activation. Conclusions. We found a statistically significant correlation between β-catenin nuclear localization and ovarian high-grade serous carcinomas. Thus, deregulation of β-catenin may play a role in the pathogenesis of ovarian high-grade serous carcinomas in contrast to ovarian low-grade serous carcinomas and LMP serous tumors.

Original languageEnglish (US)
Pages (from-to)363-368
Number of pages6
JournalGynecologic oncology
Volume88
Issue number3
DOIs
StatePublished - Mar 1 2003

Keywords

  • Ovary
  • Pathogenesis
  • Serous tumors
  • β-Catenin

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

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