β-Adrenergic receptor-adenylate cyclase of denervated sarcolemmal membrane

P. Blaise Smith; Stuart P. Grefrath; Stanley H. Appel

doi:10.1016/0014-4886(78)90228-5

β-Adrenergic receptor-adenylate cyclase of denervated sarcolemmal membrane

P. Blaise Smith, Stuart P. Grefrath, Stanley H. Appel

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Five days after motor denervation, mammalian skeletal muscle sarcolemma undergoes a 50% decline in basal and catecholamine-stimulated adenylate cyclase activity. Sodium fluoride- and 5′-guanylyl imidophosphate [Gpp(NH)p)]-stimulated activities also are depressed. However, properties of the β-adrenergic receptor in the plasma membrane preparation of denervated muscle are similar to those of normal innervated muscle. The number and affinity of specific (-)-[³H]dihydroalprenolol binding sites, the effects of catecholaminergic ligands on binding (isoproterenol > epinephrine > norepinephrine), the stimulatory effects of Gpp(NH)p on adenylate cyclase activity, and the shift in concentration of catecholamines required to activate adenylate cyclase in the presence of Gpp(NH)p were similar in normal and denervated membranes. Thus denervation appears to uncouple the adenylate cyclase response from β-adrenergic stimulation primarily by a loss of adenylate cyclase activity with no change in receptor properties.

Original language	English (US)
Pages (from-to)	361-371
Number of pages	11
Journal	Experimental Neurology
Volume	59
Issue number	3
DOIs	https://doi.org/10.1016/0014-4886(78)90228-5
State	Published - May 1 1978

ASJC Scopus subject areas

Neurology
Developmental Neuroscience

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title = "β-Adrenergic receptor-adenylate cyclase of denervated sarcolemmal membrane",

abstract = "Five days after motor denervation, mammalian skeletal muscle sarcolemma undergoes a 50% decline in basal and catecholamine-stimulated adenylate cyclase activity. Sodium fluoride- and 5′-guanylyl imidophosphate [Gpp(NH)p)]-stimulated activities also are depressed. However, properties of the β-adrenergic receptor in the plasma membrane preparation of denervated muscle are similar to those of normal innervated muscle. The number and affinity of specific (-)-[3H]dihydroalprenolol binding sites, the effects of catecholaminergic ligands on binding (isoproterenol > epinephrine > norepinephrine), the stimulatory effects of Gpp(NH)p on adenylate cyclase activity, and the shift in concentration of catecholamines required to activate adenylate cyclase in the presence of Gpp(NH)p were similar in normal and denervated membranes. Thus denervation appears to uncouple the adenylate cyclase response from β-adrenergic stimulation primarily by a loss of adenylate cyclase activity with no change in receptor properties.",

author = "Smith, {P. Blaise} and Grefrath, {Stuart P.} and Appel, {Stanley H.}",

note = "Funding Information: Previous work from our laboratory characterized the ,8-adrenergic receptor-adenylate cyclase system in the sarcolemmal membrane isolated l This paper is dedicated to the memory of Dr. Stuart P. Grefrath, deceased. We thank Mrs. Tommye Campbell for expert secretarial assistance. This work was supported by Grant NS-07872 from the National Institutes of Health. Portions of this study were presented by Dr. P. Blaise Smith at the annual FASEB meeting, San Francisco, California, June 1976, and Dr. Stuart P. Grefrath at the annual meeting of the Society for Neuroscience, Toronto, Ontario, Canada, November 1976. Requests for reprints should be addressed to Dr. Appel at Baylor College of Medicine. Abbreviations : Gpp (NH) p--5{\textquoteright}-guanylyl imidodiphosphate ; CAMP-adenosine 3{\textquoteright} : 5{\textquoteright}-cyclic monophosphate. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1978",

month = may,

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doi = "10.1016/0014-4886(78)90228-5",

language = "English (US)",

volume = "59",

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T1 - β-Adrenergic receptor-adenylate cyclase of denervated sarcolemmal membrane

AU - Smith, P. Blaise

AU - Grefrath, Stuart P.

AU - Appel, Stanley H.

N1 - Funding Information: Previous work from our laboratory characterized the ,8-adrenergic receptor-adenylate cyclase system in the sarcolemmal membrane isolated l This paper is dedicated to the memory of Dr. Stuart P. Grefrath, deceased. We thank Mrs. Tommye Campbell for expert secretarial assistance. This work was supported by Grant NS-07872 from the National Institutes of Health. Portions of this study were presented by Dr. P. Blaise Smith at the annual FASEB meeting, San Francisco, California, June 1976, and Dr. Stuart P. Grefrath at the annual meeting of the Society for Neuroscience, Toronto, Ontario, Canada, November 1976. Requests for reprints should be addressed to Dr. Appel at Baylor College of Medicine. Abbreviations : Gpp (NH) p--5’-guanylyl imidodiphosphate ; CAMP-adenosine 3’ : 5’-cyclic monophosphate. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1978/5/1

Y1 - 1978/5/1

N2 - Five days after motor denervation, mammalian skeletal muscle sarcolemma undergoes a 50% decline in basal and catecholamine-stimulated adenylate cyclase activity. Sodium fluoride- and 5′-guanylyl imidophosphate [Gpp(NH)p)]-stimulated activities also are depressed. However, properties of the β-adrenergic receptor in the plasma membrane preparation of denervated muscle are similar to those of normal innervated muscle. The number and affinity of specific (-)-[3H]dihydroalprenolol binding sites, the effects of catecholaminergic ligands on binding (isoproterenol > epinephrine > norepinephrine), the stimulatory effects of Gpp(NH)p on adenylate cyclase activity, and the shift in concentration of catecholamines required to activate adenylate cyclase in the presence of Gpp(NH)p were similar in normal and denervated membranes. Thus denervation appears to uncouple the adenylate cyclase response from β-adrenergic stimulation primarily by a loss of adenylate cyclase activity with no change in receptor properties.

AB - Five days after motor denervation, mammalian skeletal muscle sarcolemma undergoes a 50% decline in basal and catecholamine-stimulated adenylate cyclase activity. Sodium fluoride- and 5′-guanylyl imidophosphate [Gpp(NH)p)]-stimulated activities also are depressed. However, properties of the β-adrenergic receptor in the plasma membrane preparation of denervated muscle are similar to those of normal innervated muscle. The number and affinity of specific (-)-[3H]dihydroalprenolol binding sites, the effects of catecholaminergic ligands on binding (isoproterenol > epinephrine > norepinephrine), the stimulatory effects of Gpp(NH)p on adenylate cyclase activity, and the shift in concentration of catecholamines required to activate adenylate cyclase in the presence of Gpp(NH)p were similar in normal and denervated membranes. Thus denervation appears to uncouple the adenylate cyclase response from β-adrenergic stimulation primarily by a loss of adenylate cyclase activity with no change in receptor properties.

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β-Adrenergic receptor-adenylate cyclase of denervated sarcolemmal membrane

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