Research interests

The Kiss RNA lab currently focuses on two main areas of research.  Namely, developing novel RNA Therapeutics to treat human disease and performing experiments to understand fundamental mechanisms in RNA molecular biology. 

RNA Therapeutics:

As we are housed inside the Houston Methodist Research Institute (HMRI), the lab is committed to the translation of basic science discoveries to benefit human health. Shortly after the lab opened, we joined the HMRI’s RNA Therapeutics program and began working on our first therapeutic candidate, an RNA designed to counter genes driving oncogenic transformation and cell migration in certain breast cancers.  That work has earned Dr. Kiss a Career Development Award from The American Society for Gene and Cell Therapy.  https://www.asgct.org/research/news/october-2019/asgct-career-development-awards

COVID-19 updates:

We are applying our expertise and experience with designing and developing novel RNA therapeutics to the address the urgent need COVID-19 pandemic.  Our lab is providing all needed support as HMRI’s RNA Core develops an mRNA-based COVID-19 vaccine. https://www.houstonmethodist.org/-/media/pdf/pr/covid/HM-COVID-19-Vaccine-Research-Press-Release-03172020.ashx

In addition to providing support for the vaccine effort mentioned above, we are developing three candidate RNAs designed to treat active COVID-19 disease.  These RNA therapeutic candidates are designed to slow the course of the infection by interfering with the virus's replication machinery.  This work is in its early stages as the required constructs and reagents have either just arrived or are scheduled to arrive by the middle of April. 

RNA molecular biology:

The basic science research interests of the lab lie in the changes that occur in the RNA and molecular biology of cells when cellular stress responses converge to cause or exacerbate cardiovascular disease or cancer. I am building a two-pronged collaborative group that leverages RNA molecular biology tools with both specialized and traditional RNA sequencing approaches combined with long-read sequencing to elucidate how these RNA-mediated changes occur. For cytoplasmic RNA recapping, my work aims to determine the regulators that determine the conditions under which, and position(s) where, an RNA is recapped in the cytoplasm. My lab uses both transcriptome-wide (microarrays, RNA-seq and ribosome profiling) and targeted methods (qPCR, polysome gradients, etc.) to understand how cytoplasmic capping drives oncogenic transformation and stress responses linked to cardiovascular disease. Ultimately, I aim to uncover the evolutionary role of cytoplasmic RNA recapping, and to decipher the mechanism(s) controlling the selection, generation, and regulation of recapping sites, and to develop cytoplasmic recapping-based drug responsiveness screens and/or RNA therapeutics. The other part of my lab focuses on how the FHIT tumor suppressor modulates the translation of the transcriptome in cancer. My work has shown that expression of the FHIT protein results in translational changes for several known cancer-linked mRNAs. Further, that translational regulation is often driven by the 5’ translation leader sequence of the mRNA. For my future FHIT research, I plan to build upon my recently published works by expanding ribosome profiling into FHIT negative patient tumor samples and by developing better cell lines where FHIT expression is more tightly regulated.

(updated 4-8-2020)

Keywords

    Research Area Keywords

  • Heart & Vascular
  • Cancer
  • Molecular Medicine

    Free-text keywords

  • Cytoplasmic capping, FHIT, RNA biology, Ribosome profiling, stress response, Cancer research, Cardiovascular disease, translational control, next generation sequencing

Fingerprint

Messenger RNA
Concept: Messenger RNA
Weight: 100%
Domain: Medicine & Life Sciences
Exosomes
Concept: Exosomes
Weight: 71%
Domain: Medicine & Life Sciences
RNA
Concept: RNA
Weight: 65%
Domain: Medicine & Life Sciences
Gene Expression
Concept: Gene Expression
Weight: 36%
Domain: Medicine & Life Sciences
Alternative Splicing
Concept: Alternative Splicing
Weight: 33%
Domain: Medicine & Life Sciences
Chromatin
Concept: Chromatin
Weight: 29%
Domain: Medicine & Life Sciences
Neoplasms
Concept: Neoplasms
Weight: 28%
Domain: Medicine & Life Sciences
Astrocytes
Concept: Astrocytes
Weight: 26%
Domain: Medicine & Life Sciences
Untranslated RNA
Concept: Untranslated RNA
Weight: 24%
Domain: Medicine & Life Sciences
Genes
Concept: Genes
Weight: 24%
Domain: Chemical Compounds
Research OutputsResearch Output authored by Daniel L. Kiss is tagged with the concept

Journals

Journal of Neurochemistry

ISSNs: 0022-3042

Wiley

Journal

3

FEBS Letters

ISSNs: 0014-5793

Elsevier

Journal

2

RNA

ISSNs: 1355-8382

Additional searchable ISSN (Electronic): 1469-9001

Cold Spring Harbor Laboratory Press

Journal

2

Molecular biology of the cell

ISSNs: 1059-1524

American Society for Cell Biology

Journal

2

Nucleic acids research

ISSNs: 0305-1048, 1362-4962

Additional searchable ISSN (Electronic): 1362-4962

Oxford University Press

Journal

1
has published Research OutputsResearch Output by Daniel L. Kiss

Research Network

Visualization: Institutional Network

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Daniel L. Kiss

Visualization: Co-author Network

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Daniel L. Kiss

Shared Pub. External Organizations

Ohio State University

External organization: Unknown

7

Western Reserve University

External organization: Unknown

4

Cleveland State University

External organization: Academic

3

Case Western Reserve University School of Medicine

External organization: Unknown

3

Elan Corporation

External organization: Corporate

3
has Research OutputsResearch Output by Daniel L. Kiss

Shared Pub. External co-authors

Erik D. Andrulis

External person

6

Ralf Bundschuh

External person

5

Daniel R. Schoenberg

External person

5

K. Huebner

External person

3

Sunita Gopalan

External person

3
has co-authored Research OutputsResearch Output with Daniel L. Kiss

ID: 38866097